AbstractsChemistry

Stability Studies of MOMIPP, an Inducer of Methuosis

by Zhaoqi Zhang




Institution: University of Toledo Health Science Campus
Department: Medicinal Chemistry
Degree: MS
Year: 2014
Keywords: Pharmaceuticals; MOMIPP, HPLC, LC-MS, Stability tests
Record ID: 2026619
Full text PDF: http://rave.ohiolink.edu/etdc/view?acc_num=mco1418402908


Abstract

Recently, “methuosis” has been identified and labeled as a novel type of cell death. One of the most potent inducers of methuosis is 3-(5-methoxy-2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2-propen-1-one which is abbreviated as “MOMIPP”. MOMIPP is being developed as a treatment for glioblastoma. As part of MOMIPP’s development, we are evaluating its stability under several storage and exposure conditions. These include storage in DMSO or chloroform:methanol (2:1) from -80 °C to room temperature for up to 6 months. Likewise, the effects of UV/visible light exposure and storage under high levels of oxygen were assessed. We also evaluated the possibility that MOMIPP might be subject to change when undergoing several freeze-thaw procedure steps or when placed on an HPLC autosampler for prolonged periods of time. Results indicate MOMIPP is stable in DMSO at most temperatures but evaporation is a problem in chloroform-methanol (2:1) except at the lowest temperature. Continuous UV light exposure appears to cause a small amount of breakdown, while visible light does not yield any significant change. Freeze-thawing should be acceptable for up to 5 cycles and placement of samples on an HPLC autosampler should not extend past 2 days. Based on these results, optimal storage and handling conditions have been establish for MOMIPP solutions that will be used during its potential development as a new drug candidate.