AbstractsPhysics

Origin of unexpected condenser-like rings observed in Fourier plane images obtained using immersion-oil objective lenses

by Bandar Mohammed Alharbi




Institution: Texas Tech University
Department:
Year: 2015
Keywords: Optical microscope
Posted: 02/05/2017
Record ID: 2067118
Full text PDF: http://hdl.handle.net/2346/63652


Abstract

I show that different setups for bright field optical microscopy which are usually used in biomedical laboratories behave like efficient microscope condenser that are responsible for the observed sub -wavelength resolution. We present a series of experiments that reveal how inclined illumination from such unexpected condensers occurs when the sample is being perpendicularly illuminated by microscope’s built-in white -light source. In addition, using low numerical aperture objective lenses in combination with the Fourier Plane Imaging Microscopy technique, we demonstrate the possibility of fabricating an inexpensive add-on optical module that can serve as an efficient and light -weight microscope condenser. Advisors/Committee Members: Bernussi, Ayrton (committee member), Grave de Peralta, Luis (Committee Chair).