Origin of unexpected condenser-like rings observed in Fourier plane images obtained using immersion-oil objective lenses
Institution: | Texas Tech University |
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Department: | |
Year: | 2015 |
Keywords: | Optical microscope |
Posted: | 02/05/2017 |
Record ID: | 2067118 |
Full text PDF: | http://hdl.handle.net/2346/63652 |
I show that different setups for bright field optical microscopy which are usually used in biomedical laboratories behave like efficient microscope condenser that are responsible for the observed sub -wavelength resolution. We present a series of experiments that reveal how inclined illumination from such unexpected condensers occurs when the sample is being perpendicularly illuminated by microscope’s built-in white -light source. In addition, using low numerical aperture objective lenses in combination with the Fourier Plane Imaging Microscopy technique, we demonstrate the possibility of fabricating an inexpensive add-on optical module that can serve as an efficient and light -weight microscope condenser. Advisors/Committee Members: Bernussi, Ayrton (committee member), Grave de Peralta, Luis (Committee Chair).