Abstracts

Blue light (BL) exposure is considered a risk factor for the development of uveal melanoma (UM), and individuals with fair skin and light irises are at the greatest risk. Moreover, commercially available BL filtering intraocular lenses (IOLs; filter 50% of the BL spectrum) have been shown to protect against the development and progression of UM in multiple in vitro and in vivo studies. However, the angiogenic effect of BL on human UM cell lines has not yet been established. This is particularly important for UM because this tumor only metastasizes hematogenously. The purpose of this study is therefore to ascertain whether filtering lesser amounts of BL (less than 50%) will maintain the protective effect against UM development in order to customize each IOL based on individualized risk. Moreover, the effect of filtering less BL on the angiogenic properties of human UM cells will be evaluated. One human UM cells line (92.1) and one transformed uveal melanocyte cell line (UW-1) were used for all experiments. The experimental setup included a light source of 10,000 lux, infrared and ultraviolet light cut-off filters and two different commercially available BL filters of different intensities (16% and 20%). Each experiment included a control group fully exposed to the light and a condition group covered with one of the two filters. The cells were then exposed for 3 hours daily over a total period of 4 days. Cell number was then determined using a proliferation assay (cell counting kit-8; CCK-8) at the end of the 4-day period. A similar experimental setup was used to quantify levels of different pro-angiogenic factors secreted by the two cell lines using a multiplex sandwich-ELISA-based quantitative array.For the 92.1 cell line, filtering 20% of BL decreased the proliferation rate significantly compared to the control group (P <0.01). However, filtering 16% of BL was not sufficient to show this same effect. Conversely, for the UW-1 cell line, filtering 16% of BL decreased the proliferation rate significantly compared to the control group (P < 0.01). Filtering 20% of BL for 92.1 cell line and 16% for UW-1 cell line showed a non-significant decrease in the levels of various pro-angiogenic factors.The protective effect against BL-induced proliferation of UM cell lines was demonstrated using the in vitro model described herein. Current commercially available IOLs filter 50% of BL. Based on ours results, the development of different BL filtering IOLs that can be prescribed according to individual patient risk of developing UM (personalized medicine) is recommended. L'exposition rpte la lumire bleue (LB) est considre comme un facteur de risque pour le dveloppement de mlanomes de l'uve (MU). Ce risque est augment chez les individus au teint et aux iris clairs. De plus, un effet angiognique de la LB fut tabli pour des modles cellulaires humains de MU. Ceci est d'une importance particulire tant donn que les MU mtastasent par voie sanguine. Il fut dmontr dans plusieurs tudes in vitro et in vivo queAdvisors/Committee Members: Miguel Noel Burnier (Internal/Supervisor).