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Detyrosination of alpha-tubulin seems to be conserved over all eukaryotes. However, its biological function in plants has remained obscure. In order to get insight into the physiological function of tubulin detyrosination, I was phenotyping an overexpressor rice mutant, which is shifting the cycle towards the tyrosinated form. In a second approach an in vitro system was established in order to analyze what happens to microtubule polymerization if these conditions are changed outside a cell.