Autoradiographic studies of antibodies produced in vitro.
Institution: | McGill University |
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Department: | Department of Bacteriology & Immunology. |
Degree: | MS. |
Year: | 1965 |
Keywords: | Bacteriology & Immunology. |
Record ID: | 1500479 |
Full text PDF: | http://digitool.library.mcgill.ca/thesisfile118065.pdf |
The in vitro synthesis of serum proteins or specific antibodies by tissues has been the subject of several investigations (Stavitsky, 1961). Tissue culture fluids have been examined for the presence of serum proteins or antibodies by several methods, many of which have involved the use of radioisotopes. Radioactively labelled omino acids have been added to tissue culture media and subsequently the synthesized label led proteins detected. In other studies, antigens labelled with radioisotopes have been added to tissue culture fluids to detect small amounts of antibody. [...]