One of the most important issues on information systems (IS) research is the need to align business with information systems and information technology (IT). Since information systems facilitate the success of business strategies, the importance of business-IT (or strategic) alignment is unquestionable. While organisations address alignment achievement, they are continually suffering from misalignments. These difficulties (the misalignments) encumber the achievement of alignment, and lead us to the phenomenon of misalignment. This Ph.D. dissertation deals with the concept of misalignment, with special attention on enterprise architecture (EA)-based analytical potential. The main purpose of the proposed research is to analyse strategic misalignment between the business dimension and the information systems dimension. The problem of business-IT alignment is translated into the aspects of enterprise architecture. The study aims to accomplish an EA-based, systematic analysis of mismatches between business and information systems.