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Abstracts Biological Sciences
Studies on yeast in vitro protein synthesis
by Christopher Hal Sissons
| Institution: | University of Auckland |
|---|---|
| Department: | |
| Degree: | |
| Year: | 1971 |
| Keywords: | |
| Posted: | |
| Record ID: | 1312315 |
| Full text PDF: | http://hdl.handle.net/2292/844 |
Abstract
1. An in vitro protein synthesizing system was developed from a brewer's yeast (Saccharomyces cerevisiae). It was characterized, and some of the conditions necessary for optimal protein synthesis established. 2. Only a small proportion (leas than 20%) of the ribosomal population was active in polyU-directed polyphenylalanine synthesis. These active ribosomes probably derived from the in vivo 80g monomers and ribosomal subunits with the polysomal ribosanes being inactive in the cell-free system.
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