Abstracts Biological Sciences

Developing Sap Nitrate Tests for Durum Wheat and Barley, Maricopa, 1999

by E. A. Riley

The standard procedure for determining nitrogen (N) status in small grains is to sample lower stem tissue for nitrate (NO₃) analysis. The tissues are then submitted to a laboratory for analysis. Sap nitrate (NO₃) can be analyzed in the field, immediately after collecting the sample, using a Cardy meter. Guidelines for sap analysis have not yet been determined. The objectives of this study were to: (i) correlate NO₃-N in dried stem tissue with sap NO₃-N , and (ii) develop sap NO₃ test guidelines for N management in durum and feed barley. In November 1998 one variety of durum (Kronos) and one variety of feed barley (Gustoe) were planted at the Maricopa Agricultural Center. Three N rates (80, 200, and 400 lbs N/acre) were applied in four split applications. Each treatment was replicated five times in a randomized complete block design. Samples were collected from lower stems at the 3-4 leaf 2 node, flag leaf visible, and heading growth stages. Grain yields ranged from 4330 lbs/A to 6794 lbs/A for Kronos and 3220 lbs/A to 4533 lbs/A for Gustoe. Correlation coefficients between stem NO₃-N and sap NO₃-N were 0.76 for Kronos and 0.60 for Gustoe. Sap NO₃-N analysis can be used to determine N status during the season for Kronos. Results for the barley suggest at low concentrations of NO₃ in the lower stem, the Cardy meter may underestimate NO₃ concentrations. This may be due to changes in moisture content in the stem as the season progresses.